Comparison of a Novel, Rapid Chromogenic Biochemical Assay, the Carba NP Test, with the Modified Hodge Test for Detection of Carbapenemase-Producing Gram-Negative Bacilli

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Evaluation of the Rapidec Carba NP Test Kit for Detection of Carbapenemase-Producing Gram-Negative Bacteria.

Recently, bioMérieux, France, introduced the Rapidec Carba NP test kit for rapid detection of carbapenemase-producing Gram-negative bacteria. This kit was evaluated in this study, and we report sensitivity, specificity, and positive and negative predictive values of 92.6%, 96.2%, 95.83%, and 92.6%, respectively. The test was easy to perform and interpret and relatively inexpensive ($5/Rs 300 pe...

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Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers.

Performances of the Rapidec Carba NP test (bioMérieux) were evaluated for detection of all types of carbapenemases in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa. In less than 2 h after sample preparation, it showed a sensitivity and specificity of 96%. This ready-to-use test is well adapted to the daily need for detection of carbapenemase producers in any laboratory...

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Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa.

The Carba NP test was evaluated against a panel of 244 carbapenemase- and non-carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa isolates. We confirmed the 100% specificity and positive predictive value of the test, but the sensitivity and negative predictive value were 72.5% and 69.2%, respectively, and increased to 80% and 77.3%, respectively, using a more concentrated bact...

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Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes

Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from pa...

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Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples

The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false-positive results were obtained, but false-negative results were observed with OXA-23- and G...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2013

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.00965-13